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动物药学:猪流行性腹泻病毒的分离鉴定


全文字数:12000字左右  原创时间:<=2022年

【内容摘要】

猪流行性腹泻病毒的分离鉴定

猪流行性腹泻(Porcine Epidemic Diahorrea,PED)是由猪流行性腹泻病毒(PEDV)引起的猪的一种高度接触性肠道传染病,以腹泻、呕吐和脱水为主要特证的猪肠道传染病。各种年龄的猪都易感,尤其是哺乳仔猪受害最严重,1971年首次在英国发现PED,主要引起架子猪和育肥猪群急性腹泻,当时被称为“流行性病毒性腹泻(epidemic virus diahorrea EVD)”。此后,比利时、德国、加拿大、匈牙利、法国、日本、保加利亚、瑞士等许多国家均有本病的报道,我国从1976开始陆续有本病的发生。该病在许多国家,如荷兰、德国、法国、匈牙利、前苏联以及前西德被描述。日本和中国台湾也证实有该病。本实验通过取用吉林大学小动物医院临床症状表现为疑似PED的患病猪病料。使用VERO细胞培养物对病毒进行分离试验, 获得满意结果。
关键词:猪流行性腹泻  分离鉴定 

ISOLATION AND IDENTIFICATION OF PORCINE EPIDEMIC DIAHORREA VIRUS
 
Abstract:Porcine epidemic diarrhea (Porcine Epidemic Diahorrea, PED), a highly contagious swine intestinal diseases, is caused by Porcine epidemic diarrhea virus .diarrhea, vomiting and dehydration are the main Clinical symptoms of PED,Pigs of all ages are susceptible, especially the worst affected piglets, It  was discovered the first time in the UK for the first time in 1971. Since then, Belgium, Germany, Canada, Hungary, France, Japan, Bulgaria, Switzerland and many other countries have reported the disease consequently.It was also  be found in china in1976. Disease in many countries, such as the Netherlands, Germany, France, Hungary, the former Soviet Union and the former West Germany is described. Japan and China Taiwan have confirmed had this disease. In this study, the small animal hospital of Jilin University offer the clinical symptoms of the prevalence of suspected PED swine feed.VERO cell cultures used to separate the virus tests and got satisfactory results.
Key words:  Porcine Epidemic Diahorrea, isolation and identification

 

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