原子吸收法测定多种蔬菜中的铁化学结构元素是构成一个人体的基本原料，化学教学元素在人体生长、发育、衰老、疾病、死亡中也起着非常重要的作用，尤其是微量元素。微量元素参与人体内各种酶、辅酶、维生素、激素和核酸的合成，具有特殊的生理功能，与细胞增值和新陈代谢密切相关。人体所需的微量元素主要来自食物和饮用水。本文对蔬菜中铁的含量影响进行了分析研究。 本文认为，蔬菜的铁含量是由原子吸收决定的，该方法是测定微量铁含量常用的方法，该方法具有选择性高、拥有属性快速、简便、灵敏度高、分析范围广等特点。样品选用生活中便宜且常见的蔬菜，用硝酸在加热技术条件下破坏菜蔬样品中的有机物及还原性研究物质，消除有机物和悬浮物对样品测定的干扰，将各种价态的铁氧化成单一的高价态形式或转化成容易分离的无机化合物，同时样液得到浓缩。 关键词：铁；蔬菜；原子吸收；硝酸 Abstract Chemical elements are not only the basic raw materials of human body, but also play a very important role in human growth, development, aging, disease and death, especially trace elements. Trace elements are involved in the formation of various enzymes, coenzymes, vitamins, hormones and nucleic acids in human body. They have specific physiological functions and are closely related to cell proliferation and metabolism. The main sources of trace elements are food and drinking water. The content of iron in vegetables was studied in this paper. In this paper, the content of iron in vegetables was determined by atomic absorption spectrometry. This method is a common method for the determination of trace iron. It has the characteristics of strong selectivity, fast and simple determination, high sensitivity and wide range of analysis. The cheap and common vegetables in daily life were selected as the samples. Nitric acid was used to destroy the organic and reducing substances in the vegetable samples under the heating condition, so as to eliminate the interference of organic and suspended substances. The iron in various valence states was oxidized into a single high valence state or transformed into inorganic compounds which are easy to separate, and the sample solution was concentrated at the same time. Key words: iron; vegetables; atomic absorption spectrometry; nitric acid 目 录 第1章 绪论 1 1.1 铁元素与人体健康 1 1.2 蔬菜中铁元素的检测方法 1 1.2.1 蔬菜样前处理 1 1.2.2 测定方法 3 1.3 主要研究内容 3 1.3.1 火焰原子吸收法测定铁的方法研究 3 1.3.2 不同蔬菜中铁元素含量的测定与比较 3 1.3.3 本论文需要解决的问题 3 第2章 实验部分 4 2.1 仪器与试剂 4 2.1.1 仪器 4 2.1.2 试剂 4 2.1.3 铁标准系列溶液的配制 4 2.1.4 仪器工作条件 4 2.2 样品预处理 5 2.2.1 蔬菜样洗涤 5 2.2.2 蔬菜样消化 5 2.3 试样测定方法 5 2.4 结果与讨论 5 2.4.1 消化最佳条件的选择 5 2.4.2 标准曲线的绘制 9 2.4.3 样品测定结果 9 2.4.4 精密度与加标回收率 10 第3章 结论 12 参考文献 13 致 谢 15
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